![]() ![]() ![]() “One, did they actually test your primer pair, and two, was it up to MIQE standards? Testing amplification efficiency without a serial dilution won’t cut it.” “If a vendor claims to have wet-tested its primers, ask two questions to ensure your real-time PCR experiment will succeed,” said Sam Ropp, Marketing Manager of Bio-Rad’s Gene Expression Division. Thus, to ensure MIQE compliance, researchers still have to wet-lab validate their assays, which can waste time and resources. While all commercial assay providers use computer-based algorithms to design primers for qPCR, this alone does not guarantee that the assay will actually work in the lab. Other companies offering “validated” primer assays simply validate their primer design algorithm and do not wet-lab test each assay to ensure that they all meet MIQE guidelines. ![]()
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